Molecular Analysis of Carbohydrate−Antibody Interactions: Case Study Using a Bacillus anthracis Tetrasaccharide
MA Oberli, M Tamborrini, YH Tsai… - Journal of the …, 2010 - ACS Publications
Journal of the American Chemical Society, 2010•ACS Publications
The process for selecting potent and effective carbohydrate antigens is not well-established.
A combination of synthetic glycan microarray screening, surface plasmon resonance
analysis, and saturation transfer difference NMR spectroscopy was used to dissect the
antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with
atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-
based design of carbohydrate antigens.
A combination of synthetic glycan microarray screening, surface plasmon resonance
analysis, and saturation transfer difference NMR spectroscopy was used to dissect the
antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with
atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-
based design of carbohydrate antigens.
The process for selecting potent and effective carbohydrate antigens is not well-established. A combination of synthetic glycan microarray screening, surface plasmon resonance analysis, and saturation transfer difference NMR spectroscopy was used to dissect the antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-based design of carbohydrate antigens.
ACS Publications