[HTML][HTML] Flow-cytometric phosphoprotein analysis reveals agonist and temporal differences in responses of murine hematopoietic stem/progenitor cells

D Kalaitzidis, BG Neel - PloS one, 2008 - journals.plos.org
D Kalaitzidis, BG Neel
PloS one, 2008journals.plos.org
Hematopoietic stem cells (HSCs) are probably the best-studied adult tissue-restricted stem
cells. Although methods for flow cytometric detection of phosphoproteins in hematopoeitic
progenitors and mature cells are available, analogous protocols for HSC are lacking. We
present a robust method to study intracellular signaling in immunophenotypically-defined
murine HSC/progenitor cell (HPC)-enriched populations. Using this method, we uncover
differences in the response dynamics of several phosphoproteins representative of the …
Hematopoietic stem cells (HSCs) are probably the best-studied adult tissue-restricted stem cells. Although methods for flow cytometric detection of phosphoproteins in hematopoeitic progenitors and mature cells are available, analogous protocols for HSC are lacking. We present a robust method to study intracellular signaling in immunophenotypically-defined murine HSC/progenitor cell (HPC)-enriched populations. Using this method, we uncover differences in the response dynamics of several phosphoproteins representative of the Ras/MAP-Kinase(K), PI3K, mTOR and Jak/STAT pathways in HSC/HPCs stimulated by Scf, Thpo, as well as several other important HSC/HPC agonists.
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