Increased serum levels and expression of S100A8/A9 complex in infiltrated neutrophils in atherosclerotic plaque of unstable angina
S Miyamoto, M Ueda, M Ikemoto, T Naruko, A Itoh… - Heart, 2008 - heart.bmj.com
S Miyamoto, M Ueda, M Ikemoto, T Naruko, A Itoh, S Tamaki, R Nohara, F Terasaki…
Heart, 2008•heart.bmj.comBackground: The S100A8/A9 complex is expressed in a subset of activated neutrophils and
macrophages in acute inflammatory lesions associated with various diseases. Objective: To
investigate (a) whether serum S100A8/A9 levels are increased in patients with unstable
angina (UA); and (b) whether S100A8/A9 expression is upregulated in coronary
atherosclerotic plaques of patients with UA. Design: Serum S100A8/A9 levels in 39 patients
with stable angina (SA) and 53 patients with UA were measured. In addition, the presence of …
macrophages in acute inflammatory lesions associated with various diseases. Objective: To
investigate (a) whether serum S100A8/A9 levels are increased in patients with unstable
angina (UA); and (b) whether S100A8/A9 expression is upregulated in coronary
atherosclerotic plaques of patients with UA. Design: Serum S100A8/A9 levels in 39 patients
with stable angina (SA) and 53 patients with UA were measured. In addition, the presence of …
Background
The S100A8/A9 complex is expressed in a subset of activated neutrophils and macrophages in acute inflammatory lesions associated with various diseases.
Objective
To investigate (a) whether serum S100A8/A9 levels are increased in patients with unstable angina (UA); and (b) whether S100A8/A9 expression is upregulated in coronary atherosclerotic plaques of patients with UA.
Design
Serum S100A8/A9 levels in 39 patients with stable angina (SA) and 53 patients with UA were measured. In addition, the presence of the S100A8/A9 complex in directional coronary atherectomy specimens was studied immunohistochemically. Cell types which stain positive for S100A8/A9 were identified by immunodouble staining with neutrophils and macrophages.
Results
Mean (SD) serum S100A8/A9 levels were significantly higher in patients with UA than in those with SA (3.25 (3.08) μg/ml vs 0.77 (0.31) μg/ml, p<0.05). In patients with UA, immunodouble staining clearly showed that the S100A8/A9 complex was expressed in infiltrated neutrophils and occasional macrophages. The S100A8/A9-positive area was significantly higher in UA than in SA (mean (SD) 18.3 (14.2)% vs 1.3 (2.4)%, respectively, p<0.001).
Conclusions
The S100A8/A9 complex may be involved in the inflammatory process of coronary atherosclerotic plaques in patients with UA.
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