Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers

I Lua, Y Li, JA Zagory, KS Wang, SW French… - Journal of …, 2016 - Elsevier
Background & Aims Contribution of hepatic stellate cells (HSCs), portal fibroblasts (PFs),
and mesothelial cells (MCs) to myofibroblasts is not fully understood due to insufficient
availability of markers and isolation methods. The present study aimed to isolate these cells,
characterize their phenotypes, and examine their contribution to myofibroblasts in liver
fibrosis. Methods Liver fibrosis was induced in Collagen1a1-green fluorescent protein
(Col1a1 GFP) mice by bile duct ligation (BDL), 3, 5-diethoxycarbonyl-1, 4-dihydrocollidine …