Production and purification of lentiviral vectors

G Tiscornia, O Singer, IM Verma - Nature protocols, 2006 - nature.com
G Tiscornia, O Singer, IM Verma
Nature protocols, 2006nature.com
Lentiviral vectors offer unique versatility and robustness as vehicles for gene delivery. They
can transduce a wide range of cell types and integrate into the host genome in both dividing
and post-mitotic cells, resulting in long-term expression of the transgene both in vitro and in
vivo. This protocol describes how lentiviral vectors can be produced, purified and titrated.
High titer suspensions can be routinely prepared with relative ease: a low-titer (106 viral
particles/ml) unpurified preparation can be obtained 3 d after transfecting cells with lentiviral …
Abstract
Lentiviral vectors offer unique versatility and robustness as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome in both dividing and post-mitotic cells, resulting in long-term expression of the transgene both in vitro and in vivo. This protocol describes how lentiviral vectors can be produced, purified and titrated. High titer suspensions can be routinely prepared with relative ease: a low-titer (106 viral particles/ml) unpurified preparation can be obtained 3 d after transfecting cells with lentiviral vector and packaging plasmids; a high-titer (109 viral particles/ml) purified preparation requires 2 more days.
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