Perilipin A is a key regulator of triacylglycerol storage and hydrolysis in adipocytes;
phosphorylation of perilipin A by protein kinase A facilitates maximal lipolysis. Chronic
stimulation of lipolysis in 3T3-L1 adipocytes causes large perinuclear lipid droplets to
fragment into myriad dispersed perilipin A-covered microlipid droplets. In cultured fibroblasts
stably expressing ectopic perilipin A, clustered lipid droplets disperse throughout the
cytoplasm upon incubation of the cells with forskolin and isobutylmethylxanthine (IBMX) to …