Erythropoietin induces the tyrosine phosphorylation of insulin receptor substrate-2: an alternate pathway for erythropoietin-induced phosphatidylinositol 3-kinase …
F Verdier, S Chrétien, C Billat, S Gisselbrecht… - Journal of Biological …, 1997 - ASBMB
In this report, we demonstrate that insulin receptor substrate-2 (IRS-2) is phosphorylated on
tyrosine following treatment of UT-7 cells with erythropoietin. We have investigated the
expression of IRS-1 and IRS-2 in several cell lines with erythroid and/or megakaryocytic
features, and we observed that IRS-2 was expressed in all cell lines tested. In contrast, we
did not detect the expression of IRS-1 in these cells. In response to erythropoietin, IRS-2 was
immediately phosphorylated on tyrosine, with maximal phosphorylation between 1 and 5 …
tyrosine following treatment of UT-7 cells with erythropoietin. We have investigated the
expression of IRS-1 and IRS-2 in several cell lines with erythroid and/or megakaryocytic
features, and we observed that IRS-2 was expressed in all cell lines tested. In contrast, we
did not detect the expression of IRS-1 in these cells. In response to erythropoietin, IRS-2 was
immediately phosphorylated on tyrosine, with maximal phosphorylation between 1 and 5 …
