Detecting oxidative modification of biomolecules with isotope dilution mass spectrometry: sensitive and quantitative assays for oxidized amino acids in proteins and …

JW Heinecke, FF Hsu, JR Crowley, SL Hazen… - Methods in …, 1999 - Elsevier
Publisher Summary This chapter describes sensitive and quantitative assays for measuring
o-tyrosine, m-tyrosine, o, o'-dityrosine, 3-nitrotyrosine, and 3-chloro-tyrosine in proteins and
tissues. The methods combine gas chromatography (GC) with isotope dilution negative-ion
electron capture mass spectrometry (MS). GC/MS has enabled to determine the relative
amounts of oxidized amino acids in proteins and lipoproteins oxidized in vitro. This
approach also quantifies levels of modified amino acids in atherosclerotic lesions …