The sequences of thousands of proteins are accessible in scientific databases and their
number is steadily growing. Protein sequences from a great number of pathogens are thus
available for mapping T-cell epitopes that may be used for immunization or immune therapy.
One of the major problems encountered in T-cell epitope mapping is the very low number of
antigen-specific T cells in peripheral blood". Therefore, usually, T-cell lines or clones have to
be established prior to performing proliferation or cytotoxicity assays. Because of its capacity …