Variability of-galactosidase A and B in different tissues of man.
E Beutler, E Guinto, W Kuhl - American Journal of Human Genetics, 1973 - ncbi.nlm.nih.gov
E Beutler, E Guinto, W Kuhl
American Journal of Human Genetics, 1973•ncbi.nlm.nih.govMATERIALS AND METHODS Human tissues were removed at autopsy, refrigerated, and
used within a few days. The a-galactosidase activity was measured using 4-
methylumbelliferyl-a-galactopyranoside, and electrophoresis was carried out at pH. 7.0 in a
1 mm phosphate buffer [3]. In some studies, electrophoresis of the enzyme was also carried
out at the same voltage in gel buffer consisting of 1 mm citrate (pH 4.5) with a 10 mm citrate
buffer (pH 4.5) in the electrode chambers.
used within a few days. The a-galactosidase activity was measured using 4-
methylumbelliferyl-a-galactopyranoside, and electrophoresis was carried out at pH. 7.0 in a
1 mm phosphate buffer [3]. In some studies, electrophoresis of the enzyme was also carried
out at the same voltage in gel buffer consisting of 1 mm citrate (pH 4.5) with a 10 mm citrate
buffer (pH 4.5) in the electrode chambers.
MATERIALS AND METHODS
Human tissues were removed at autopsy, refrigerated, and used within a few days. The a-galactosidase activity was measured using 4-methylumbelliferyl-a-galactopyranoside, and electrophoresis was carried out at pH. 7.0 in a 1 mm phosphate buffer [3]. In some studies, electrophoresis of the enzyme was also carried out at the same voltage in gel buffer consisting of 1 mm citrate (pH 4.5) with a 10 mm citrate buffer (pH 4.5) in the electrode chambers.
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