Methods for assessment of tissue sites of lipoprotein degradation
RC Pittman, CA Taylor Jr - Methods in enzymology, 1986 - Elsevier
Publisher Summary This chapter describes various methods for assessments of tissues sites
of lipoproteins degradation. At the present time the method of choice for most applications
seems to be the tyramine-cellobiose technique. Tyramine, the radioiodine acceptor, is linked
to cellobiose, a non hydrolyzable reducing disaccharide, by reductive amination. The
resulting tyramine-cellobiose adduct (TC) is then attached to the protein using cyanuric
chloride. Two biological requisites must be met for the trapped label approach to be …
of lipoproteins degradation. At the present time the method of choice for most applications
seems to be the tyramine-cellobiose technique. Tyramine, the radioiodine acceptor, is linked
to cellobiose, a non hydrolyzable reducing disaccharide, by reductive amination. The
resulting tyramine-cellobiose adduct (TC) is then attached to the protein using cyanuric
chloride. Two biological requisites must be met for the trapped label approach to be …
