Processing of human acid sphingomyelinase in normal and I-cell fibroblasts.
R Hurwitz, K Ferlinz, G Vielhaber, H Moczall… - Journal of Biological …, 1994 - ASBMB
The biosynthesis of acid sphingomyelinase in normal and I-cell disease fibroblasts was
investigated by metabolic labeling with [35S] methionine and immunoprecipitation followed
by polyacrylamide gel electrophoresis and fluorography. Two major polypeptides with
apparent molecular masses of 75 and 72 kDa (peptide chains of 64 and 61 kDa,
respectively) and a minor one with molecular mass of 57 kDa (peptide chain of 47 kDa) were
found intracellularly soon after pulse labeling. The 75-kDa form is assumed to be the …
investigated by metabolic labeling with [35S] methionine and immunoprecipitation followed
by polyacrylamide gel electrophoresis and fluorography. Two major polypeptides with
apparent molecular masses of 75 and 72 kDa (peptide chains of 64 and 61 kDa,
respectively) and a minor one with molecular mass of 57 kDa (peptide chain of 47 kDa) were
found intracellularly soon after pulse labeling. The 75-kDa form is assumed to be the …