Interleukin‐10 is localized to and released by human lung mast cells
Ishizuka, Okayama, Kobayashi… - Clinical & Experimental …, 1999 - Wiley Online Library
Ishizuka, Okayama, Kobayashi, Mori
Clinical & Experimental Allergy, 1999•Wiley Online LibraryBackground Mast cells control the local inflammation by producing many kinds of cytokines.
Interleukin (IL)‐10 is one of the important cytokine that upregulate or downregulate
inflammation. Objective The aim of this study is to ascertain whether IL‐10 is produced from
human lung mast cells by cross‐linkage of high‐affinity Fcε receptors (FcεRI). Methods Mast
cells were purified using affinity magnetic selection with mAb YB5. B8 (> 93% pure). Mast
cells were precultured with human myeloma IgE (3 μg/mL) for 16 h and then washed, and …
Interleukin (IL)‐10 is one of the important cytokine that upregulate or downregulate
inflammation. Objective The aim of this study is to ascertain whether IL‐10 is produced from
human lung mast cells by cross‐linkage of high‐affinity Fcε receptors (FcεRI). Methods Mast
cells were purified using affinity magnetic selection with mAb YB5. B8 (> 93% pure). Mast
cells were precultured with human myeloma IgE (3 μg/mL) for 16 h and then washed, and …
Background
Mast cells control the local inflammation by producing many kinds of cytokines. Interleukin (IL)‐10 is one of the important cytokine that upregulate or downregulate inflammation.
Objective
The aim of this study is to ascertain whether IL‐10 is produced from human lung mast cells by cross‐linkage of high‐affinity Fcε receptors (FcεRI).
Methods
Mast cells were purified using affinity magnetic selection with mAb YB5.B8 (> 93% pure). Mast cells were precultured with human myeloma IgE (3 μg/mL) for 16 h and then washed, and stimulated with anti‐IgE in the presence or absence of recombinant human stem cell factor (rhSCF). We have studied the production of IL‐10 by using reverse transcription‐PCR, enzyme‐linked immunosorbent assay and immunocytochemistry.
Results
We found that human lung mast cells were immunocytochemically stained with anti‐IL‐10 mAb after IgE‐dependent stimulation. The activation of mast cells via FcεRI enhanced the intensity of the IL‐10 mRNA signal. Anti‐IgE (1 μg/mL) induced a median IL‐10 release of 301.7 (7.8–1532.4) pg/106 mast cells/24 h. In contrast, mast cells released only a small amount of IL‐10 in the absence of anti‐IgE. This difference was statistically significant (P = 0.02, n = 11).
Conclusion
Our findings indicate that human lung mast cells are capable of producing IL‐10 in response to IgE‐dependent stimulation.
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