Microassay of free and total creatine from tissue extracts by combination of chromatographic and fluorometric methods

H Kammermeier - Analytical Biochemistry, 1973 - Elsevier
H Kammermeier
Analytical Biochemistry, 1973Elsevier
A method is described for specific microassay of creatine in tissue extracts. Separation by
paper-or thin-layer chromatography and a specific fluorescence reaction yielded a high
specificity. Reproducibility of total creatine assay was improved by a special procedure for
hydrolization of creatine phosphate.
A method is described for specific microassay of creatine in tissue extracts. Separation by paper- or thin-layer chromatography and a specific fluorescence reaction yielded a high specificity. Reproducibility of total creatine assay was improved by a special procedure for hydrolization of creatine phosphate.
Elsevier