[HTML][HTML] Formation of reactive cyclopentenone compounds in vivo as products of the isoprostane pathway
Y Chen, JD Morrow, LJ Roberts - Journal of Biological Chemistry, 1999 - ASBMB
Cyclopentenone prostaglandins A 2 and J 2 are reactive compounds that possess unique
biological activities. However, the extent to which they are formed in vivo remains unclear. In
this study, we explored whether D 2/E 2-isoprostanes undergo dehydration in vivo to form A
2/J 2-isoprostanes. Oxidation of arachidonic acid in vitro generated a series of compounds
that were confirmed to be A 2/J 2-isoprostanes by mass spectrometric analyses. A 2/J 2-
isoprostanes were detected in vivo esterified to lipids in livers from normal rats at a level of …
biological activities. However, the extent to which they are formed in vivo remains unclear. In
this study, we explored whether D 2/E 2-isoprostanes undergo dehydration in vivo to form A
2/J 2-isoprostanes. Oxidation of arachidonic acid in vitro generated a series of compounds
that were confirmed to be A 2/J 2-isoprostanes by mass spectrometric analyses. A 2/J 2-
isoprostanes were detected in vivo esterified to lipids in livers from normal rats at a level of …