Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor …
GK Michalopoulos, WC Bowen, VF Zajac… - Hepatology, 1999 - journals.lww.com
Hepatocytes were grown in chemically defined hepatocyte growth medium (HGM)
containing hepatocyte growth factor (HGF) and epidermal growth factor (EGF) on collagen–
coated polystyrene beads in roller bottle cultures, forming clusters of beads, and proliferating
hepatocytes and nonparenchymal cells, including fenestrated endothelium–forming
vascular structures. Desmin–positive cells surrounded hepatocytes. Collagen types I and III
were deposited in a diffuse manner whereas collagen type IV surrounded the clusters of the …
containing hepatocyte growth factor (HGF) and epidermal growth factor (EGF) on collagen–
coated polystyrene beads in roller bottle cultures, forming clusters of beads, and proliferating
hepatocytes and nonparenchymal cells, including fenestrated endothelium–forming
vascular structures. Desmin–positive cells surrounded hepatocytes. Collagen types I and III
were deposited in a diffuse manner whereas collagen type IV surrounded the clusters of the …