[HTML][HTML] RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner
SY Park, HK Avraham, S Avraham - Journal of Biological Chemistry, 2004 - ASBMB
The related adhesion focal tyrosine kinase (RAFTK), also known as Pyk2, undergoes
autophosphorylation upon its stimulation. This leads to cascades of intracellular signaling
that result in the regulation of various cellular activities. However, the molecular mechanism
of RAFTK autophosphorylation is not yet known. Using various RAFTK constructs fused with
two different tags, we found that the autophosphorylation of RAFTK was mediated by a trans-
acting mechanism, not a cis-acting mechanism. In addition, overexpression of kinase …
autophosphorylation upon its stimulation. This leads to cascades of intracellular signaling
that result in the regulation of various cellular activities. However, the molecular mechanism
of RAFTK autophosphorylation is not yet known. Using various RAFTK constructs fused with
two different tags, we found that the autophosphorylation of RAFTK was mediated by a trans-
acting mechanism, not a cis-acting mechanism. In addition, overexpression of kinase …