[HTML][HTML] Oscillations of phospholipase C activity triggered by depolarization and Ca2+ influx in insulin-secreting cells
S Thore, O Dyachok, A Tengholm - Journal of Biological Chemistry, 2004 - ASBMB
Phospholipase C (PLC) is a ubiquitous enzyme involved in the regulation of a variety of
cellular processes. Its dependence on Ca 2+ is well recognized, but it is not known how PLC
activity is affected by physiological variations of the cytoplasmic Ca 2+ concentration ([Ca
2+] i). Here, we applied evanescent wave microscopy to monitor PLC activity in parallel with
[Ca 2+] i in individual insulin-secreting INS-1 cells using the phosphatidylinositol 4, 5-
bisphosphate-and inositol 1, 4, 5-trisphosphate-binding pleckstrin homology domain from …
cellular processes. Its dependence on Ca 2+ is well recognized, but it is not known how PLC
activity is affected by physiological variations of the cytoplasmic Ca 2+ concentration ([Ca
2+] i). Here, we applied evanescent wave microscopy to monitor PLC activity in parallel with
[Ca 2+] i in individual insulin-secreting INS-1 cells using the phosphatidylinositol 4, 5-
bisphosphate-and inositol 1, 4, 5-trisphosphate-binding pleckstrin homology domain from …