[HTML][HTML] Site of docking and fusion of insulin secretory granules in live MIN6 β cells analyzed by TAT-conjugated anti-syntaxin 1 antibody and total internal reflection …
M Ohara-Imaizumi, C Nishiwaki, T Kikuta… - Journal of Biological …, 2004 - ASBMB
To determine the site of insulin exocytosis in the pancreatic β cell plasma membrane, we
analyzed the interaction between the docking/fusion of green fluorescent protein-tagged
insulin granules and syntaxin 1 labeled by TAT-conjugated Cy3-labeled antibody (Ab) using
total internal reflection fluorescence microscopy (TIRFM). Monoclonal Ab against syntaxin 1
was labeled with Cy3 then conjugated with the protein transduction domain of HIV-1 TAT.
TAT-conjugated Cy3-labeled anti-syntaxin 1 Ab was transduced rapidly into the …
analyzed the interaction between the docking/fusion of green fluorescent protein-tagged
insulin granules and syntaxin 1 labeled by TAT-conjugated Cy3-labeled antibody (Ab) using
total internal reflection fluorescence microscopy (TIRFM). Monoclonal Ab against syntaxin 1
was labeled with Cy3 then conjugated with the protein transduction domain of HIV-1 TAT.
TAT-conjugated Cy3-labeled anti-syntaxin 1 Ab was transduced rapidly into the …