Initiation of membranal lipid peroxidation by activated metmyoglobin and methemoglobin
J Kanner, S Harel - Archives of Biochemistry and Biophysics, 1985 - Elsevier
J Kanner, S Harel
Archives of Biochemistry and Biophysics, 1985•ElsevierThe interaction of hydrogen peroxide (H 2 O 2) with metmyoglobin (MetMb) led very rapidly
to the generation of an active species which could initiate lipid peroxidation. The activity of
this prooxidant decreased rapidly during the first minutes, but 50% of its activity remained
stable for more than 30 min. In this model system, it was found that small amounts of H 2 O 2
(1–10 μ m) could activate MetMb for significant lipid peroxidation. The incubation of the
sarcosomal lipids with activated MetMb caused oxygen absorption. No absorption of oxygen …
to the generation of an active species which could initiate lipid peroxidation. The activity of
this prooxidant decreased rapidly during the first minutes, but 50% of its activity remained
stable for more than 30 min. In this model system, it was found that small amounts of H 2 O 2
(1–10 μ m) could activate MetMb for significant lipid peroxidation. The incubation of the
sarcosomal lipids with activated MetMb caused oxygen absorption. No absorption of oxygen …
Abstract
The interaction of hydrogen peroxide (H2O2) with metmyoglobin (MetMb) led very rapidly to the generation of an active species which could initiate lipid peroxidation. The activity of this prooxidant decreased rapidly during the first minutes, but 50% of its activity remained stable for more than 30 min. In this model system, it was found that small amounts of H2O2 (1–10 μm) could activate MetMb for significant lipid peroxidation. The incubation of the sarcosomal lipids with activated MetMb caused oxygen absorption. No absorption of oxygen was determined in the presence of membrane with MetMb or H2O2 alone. Methemoglobin (MetHb) was also found to be activated by H2O2 and to initiate lipid peroxidation. Membranal lipid peroxidation initiated by activated MetMb was inhibited by several reducing compounds and antioxidants. However, several hydroxyl radical scavengers and catalase failed to inhibit this reaction.
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