[HTML][HTML] Selective disruption of lysosomes in HeLa cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins
T Cirman, K Orešić, GD Mazovec, V Turk… - Journal of Biological …, 2004 - ASBMB
Increasing evidence suggests that lysosomal proteases are actively involved in apoptosis.
Using HeLa cells as the model system, we show that selective lysosome disruption with l-
leucyl-l-leucine methyl ester results in apoptosis, characterized by translocation of lysosomal
proteases into the cytosol and by the cleavage of a proapoptotic Bcl-2-family member Bid.
Apoptosis and Bid cleavage, but not translocation of lysosomal proteases to the cytosol,
could be prevented by 15 μml-trans-epoxysuccinyl (OEt)-Leu-3-methylbutylamide, an …
Using HeLa cells as the model system, we show that selective lysosome disruption with l-
leucyl-l-leucine methyl ester results in apoptosis, characterized by translocation of lysosomal
proteases into the cytosol and by the cleavage of a proapoptotic Bcl-2-family member Bid.
Apoptosis and Bid cleavage, but not translocation of lysosomal proteases to the cytosol,
could be prevented by 15 μml-trans-epoxysuccinyl (OEt)-Leu-3-methylbutylamide, an …