[HTML][HTML] Transcriptional regulation of the human monocyte chemoattractant protein-1 gene: cooperation of two NF-κB sites and NF-κB/Rel subunit specificity
A Ueda, Y Ishigatsubo, T Okubo… - Journal of Biological …, 1997 - ASBMB
Human monocyte chemoattractant protein-1 (human MCP-1) mRNA accumulated in THP-1
cells 2 h after lipopolysaccharide (LPS) stimulation. DNase I footprinting revealed that LPS
stimulation induced protein binding to the two closely located NF-κB sites, A1 and A2. By
electrophoretic gel mobility shift assay and supershift assay, the binding of (p65) 2, c-
Rel/p65, p50/p65, and p50/c-Rel to the A2 oligonucleotide probe was detected after LPS
stimulation. In contrast, 12-o-tetradecanoylphorbol 13-acetate did not induce a significant …
cells 2 h after lipopolysaccharide (LPS) stimulation. DNase I footprinting revealed that LPS
stimulation induced protein binding to the two closely located NF-κB sites, A1 and A2. By
electrophoretic gel mobility shift assay and supershift assay, the binding of (p65) 2, c-
Rel/p65, p50/p65, and p50/c-Rel to the A2 oligonucleotide probe was detected after LPS
stimulation. In contrast, 12-o-tetradecanoylphorbol 13-acetate did not induce a significant …