[HTML][HTML] Tumor necrosis factor-α inhibits endothelial nitric-oxide synthase gene promoter activity in bovine aortic endothelial cells
HDI Anderson, D Rahmutula, DG Gardner - Journal of Biological Chemistry, 2004 - ASBMB
Tumor necrosis factor-α (TNF-α) has been shown to reduce endothelial nitric-oxide synthase
(eNOS) gene expression through post-transcriptional regulation of mRNA stability. The
current study documented an independent effect of the cytokine on the eNOS gene
promoter. TNF-α effected a time-and dose-dependent reduction in activity of a transiently
transfected human–1197 eNOS-luciferase reporter. This reduction was inhibited by co-
transfection of dominant negative IKKβ as well as a nonphosphorylatable constitutively …
(eNOS) gene expression through post-transcriptional regulation of mRNA stability. The
current study documented an independent effect of the cytokine on the eNOS gene
promoter. TNF-α effected a time-and dose-dependent reduction in activity of a transiently
transfected human–1197 eNOS-luciferase reporter. This reduction was inhibited by co-
transfection of dominant negative IKKβ as well as a nonphosphorylatable constitutively …
