Multiple classes of sulfhydryls modulate the skeletal muscle Ca2+ release channel
B Aghdasi, JZ Zhang, Y Wu, MB Reid… - Journal of Biological …, 1997 - ASBMB
Two sulfhydryl reagents, N-ethylmaleimide (NEM), an alkylating agent, and diamide, an
oxidizing agent, were examined for effects on the skeletal muscle Ca 2+ release channel.
NEM incubated with the channel for increasing periods of time displays three distinct phases
in its functional effects on the channel reconstituted into planar lipid bilayers; first it inhibits,
then it activates, and finally it again inhibits channel activity. NEM also shows a three-phase
effect on the binding of [3 H] ryanodine by first decreasing binding (phase 1), followed by a …
oxidizing agent, were examined for effects on the skeletal muscle Ca 2+ release channel.
NEM incubated with the channel for increasing periods of time displays three distinct phases
in its functional effects on the channel reconstituted into planar lipid bilayers; first it inhibits,
then it activates, and finally it again inhibits channel activity. NEM also shows a three-phase
effect on the binding of [3 H] ryanodine by first decreasing binding (phase 1), followed by a …