[HTML][HTML] Topoisomerase II· etoposide interactions direct the formation of drug-induced enzyme-DNA cleavage complexes
DA Burden, PS Kingma, SJ Froelich-Ammon… - Journal of Biological …, 1996 - ASBMB
Topoisomerase II is the target for several highly active anticancer drugs that induce cell
death by enhancing enzyme-mediated DNA scission. Although these agents dramatically
increase levels of nucleic acid cleavage in a site-specific fashion, little is understood
regarding the mechanism by which they alter the DNA site selectivity of topoisomerase II.
Therefore, a series of kinetic and binding experiments were carried out to determine the
mechanistic basis by which the anticancer drug, etoposide, enhances cleavage complex …
death by enhancing enzyme-mediated DNA scission. Although these agents dramatically
increase levels of nucleic acid cleavage in a site-specific fashion, little is understood
regarding the mechanism by which they alter the DNA site selectivity of topoisomerase II.
Therefore, a series of kinetic and binding experiments were carried out to determine the
mechanistic basis by which the anticancer drug, etoposide, enhances cleavage complex …