T cells of the human intestinal lamina propria are high producers of interleukin-10
J Braunstein, L Qiao, F Autschbach, G Schürmann… - Gut, 1997 - gut.bmj.com
J Braunstein, L Qiao, F Autschbach, G Schürmann, S Meuer
Gut, 1997•gut.bmj.comBackground and aim—Some of the recently observed functional features characteristic of
immunocompetent cells residing in the human intestinal lamina propria could be mediated
by interleukin-10 (IL-10). To investigate the role of IL-10 in the human intestinal mucosa, the
regulation of IL-10 production by lamina propria T lymphocytes (LPL-T) was determined and
compared with that of peripheral blood T lymphocytes (PBL-T). Methods—Following
activation by using different stimuli, IL-10 release by LPL-T and PBL-T into the supernatant …
immunocompetent cells residing in the human intestinal lamina propria could be mediated
by interleukin-10 (IL-10). To investigate the role of IL-10 in the human intestinal mucosa, the
regulation of IL-10 production by lamina propria T lymphocytes (LPL-T) was determined and
compared with that of peripheral blood T lymphocytes (PBL-T). Methods—Following
activation by using different stimuli, IL-10 release by LPL-T and PBL-T into the supernatant …
Background and aim —Some of the recently observed functional features characteristic of immunocompetent cells residing in the human intestinal lamina propria could be mediated by interleukin- 10 (IL-10). To investigate the role of IL-10 in the human intestinal mucosa, the regulation of IL-10 production by lamina propria T lymphocytes (LPL-T) was determined and compared with that of peripheral blood T lymphocytes (PBL-T).
Methods —Following activation by using different stimuli, IL-10 release by LPL-T and PBL-T into the supernatant was measured by enzyme linked immunosorbent assay (ELISA). In parallel, cell growth was determined by [3H]-thymidine incorporation.
Results —Neither LPL-T nor PBL-T release IL-10 constitutively. Triggering through CD2 or the T cell receptor (TCR)/CD3 complex in the presence of autologous monocytes induces significantly greater IL-10 secretion by LPL-T than by PBL-T. Engagement of the CD45 receptor enhances IL-10 release and proliferation of CD2 triggered CD45RO+ PBL-T. In contrast, it reduces CD2 induced IL-10 production by LPL-T without altering cell growth significantly .
Conclusions—Activated LPL-T release relatively high amounts of IL-10. Enhanced IL-10 production by activated LPL-T, in comparison with activated PBL-T, is not only related to the presence of a higher proportion of CD45RO+ T cells in the intestinal lamina propria, but is also caused by increased sensitivity of LPL-T to CD2 co-stimulation. The differential responsiveness of LPL-T, compared with PBL-T, to CD45 engagement demonstrates that CD45 could be involved in the altered CD2 reactivity of LPL-T.
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