Mitotically synchronized mammalian cells: a simple method for obtaining large populations

E Robbins, PI Marcus - Science, 1964 - science.org
E Robbins, PI Marcus
Science, 1964science.org
Mitotically Synchronized Mammalian Cells: A Simple Method for Obtaining Large Populations
Page 1 Table 1. Rectal glands of fresh-water and marine sharks. The fresh-water species were
collected at Lake Nicaragua and Rio San Juan in June and July 1963, the marine species at
Cape Haze, July to November 1963. Ani- Body, Rectal gland mal Sex length Length Weight
No. (cm) (cm) (g) Fresh water: C. leucas 1 F* 170 4.5 1.1 2 M* 158.4 6.5 0.6 3 Ft 188.2 6.5
1.5 4 Mt 174 6.7 0.6 5 Ft 155 6.0 0.8 6 Mt 147.5 6.4 0.7 7 F§ 127.2 11 6.0 0.35 8 F§ 135 3.8 …
Eliminating most of the ionic calcium from growth medium does not affect cell growth, but it allows simple and preferential detachment of HeLa cells in mitosis from nearly confluent monolayers.
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