Changes in the localization and content of opsin during retinal development in the rds mutant mouse: immunocytochemistry and immunoassay
J Usukura, D Bok - Experimental eye research, 1987 - Elsevier
Electron-microscope immunocytochemistry and antibody staining of nitrocellulose replicas
of SDS gels (Western blots) were used in a developmental study to detect the presence and
localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their
BALB/c normal controls. Western blot analysis of isolated retinal membranes first detected
opsin at 10 postnatal days in both strains. Opsin levels rose progressively with development
in BALB/c normal retinas. In contrast, levels in the rds retina became undetectable by 30 …
of SDS gels (Western blots) were used in a developmental study to detect the presence and
localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their
BALB/c normal controls. Western blot analysis of isolated retinal membranes first detected
opsin at 10 postnatal days in both strains. Opsin levels rose progressively with development
in BALB/c normal retinas. In contrast, levels in the rds retina became undetectable by 30 …