A Ca2+-insensitive form of fura-2 associated with polymorphonuclear leukocytes. Assessment and accurate Ca2+ measurement.
M Scanlon, DA Williams, FS Fay - Journal of Biological Chemistry, 1987 - ASBMB
The new, fluorescent Ca2+ indicator, fura-2, promises to expand our understanding of the
role of subcellular changes in Ca2+ underlying cell function. During an investigation of the
role of Ca2+ in the polarization response of human polymorphonuclear leukocytes to formyl-
methionyl-leucyl-phenylalanine, we found that fura-2 trapped by cells incubated with the
acetoxy-methyl ester of fura-2, F2-AM, yielded measurements of Ca2+ that were depressed
at rest and during the response to formyl-methionyl-leucyl-phenylalanine. Fura-2, trapped by …
role of subcellular changes in Ca2+ underlying cell function. During an investigation of the
role of Ca2+ in the polarization response of human polymorphonuclear leukocytes to formyl-
methionyl-leucyl-phenylalanine, we found that fura-2 trapped by cells incubated with the
acetoxy-methyl ester of fura-2, F2-AM, yielded measurements of Ca2+ that were depressed
at rest and during the response to formyl-methionyl-leucyl-phenylalanine. Fura-2, trapped by …