Cyclic AMP-mediated phosphorylation and insolubilization of a 49-kDa cytoskeletal marker protein of lens fiber terminal differentiation
ME Ireland, C Klettner, W Nunlee - Experimental eye research, 1993 - Elsevier
Terminal differentiation of chick lens fiber cells has been previously characterized by the
accumulation, acidification via phosphorylation, and increased membrane association of a
49-kDa cytoskeletal protein. In these studies, we examine:(1) the subcellular distribution of
the 49-kDa protein with regard to ageing and isoform composition; and (2) potential
mechanisms regulating 49-kDa phosphorylation and insolubilization. With conventional
Western blotting techniques, the 49-kDa protein is found exclusively in insoluble form within …
accumulation, acidification via phosphorylation, and increased membrane association of a
49-kDa cytoskeletal protein. In these studies, we examine:(1) the subcellular distribution of
the 49-kDa protein with regard to ageing and isoform composition; and (2) potential
mechanisms regulating 49-kDa phosphorylation and insolubilization. With conventional
Western blotting techniques, the 49-kDa protein is found exclusively in insoluble form within …
