We previously showed the selective expression of the chondroitin sulfate proteoglycans
versican V0 and V1 in barrier tissues that impede the migration of neural crest cells during
embryonic trunk development (Landolt, RM, Vaughan, L., Winterhalter, KH, and
Zimmermann, DR (1995) Development 212, 2303-2312). To test for an active involvement of
these isoforms in the guidance process, we have now established protocols to isolate intact
versican V0 and V1 in quantities sufficient for functional experiments. Using stripe choice …