Improved microfluorometric DNA determination in biological material using 33258 Hoechst
CF Cesarone, C Bolognesi, L Santi - Analytical biochemistry, 1979 - Elsevier
CF Cesarone, C Bolognesi, L Santi
Analytical biochemistry, 1979•ElsevierA simple and rapid microfluorometric method is described for the determination of DNA in
submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of
reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and
HeLa cells homogenate preparations. None or very little interference by the routinely used
preparation reagents or by the cellular components was found. Compared to other
commonly used procedures this innovative and versatile technique can be conveniently …
submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of
reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and
HeLa cells homogenate preparations. None or very little interference by the routinely used
preparation reagents or by the cellular components was found. Compared to other
commonly used procedures this innovative and versatile technique can be conveniently …
A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.
Elsevier