Human ferrochelatase is an iron-sulfur protein
HA Dailey, MG Finnegan, MK Johnson - Biochemistry, 1994 - ACS Publications
HA Dailey, MG Finnegan, MK Johnson
Biochemistry, 1994•ACS PublicationsMATERIALS AND METHODS Recombinant human ferrochelatase and the carboxyl-terminal
truncated version of the enzyme were produced and purified as described previously (Dailey
et al., 1994). Ex-pression vectors for production of yeast and E. coli ferro-chelatase in E. coli
were based upon the tac promoter vector used for humanferrochelatase. These enzymes
are also purified inthe same fashion as described for human ferro-chelatase. For the
experiments described below all purification procedures were carried out within 1 day, and …
truncated version of the enzyme were produced and purified as described previously (Dailey
et al., 1994). Ex-pression vectors for production of yeast and E. coli ferro-chelatase in E. coli
were based upon the tac promoter vector used for humanferrochelatase. These enzymes
are also purified inthe same fashion as described for human ferro-chelatase. For the
experiments described below all purification procedures were carried out within 1 day, and …
MATERIALS AND METHODS
Recombinant human ferrochelatase and the carboxyl-terminal truncated version of the enzyme were produced and purified as described previously (Dailey et al., 1994). Ex-pression vectors for production of yeast and E. coli ferro-chelatase in E. coli were based upon the tac promoter vector used for humanferrochelatase. These enzymes are also purified inthe same fashion as described for human ferro-chelatase. For the experiments described below all purification procedures were carried out within 1 day, and spectra were recorded immediately. Ferrochelatase activity was determined
ACS Publications