The cytokine interleukin-1β reduces the docking and fusion of insulin granules in pancreatic β-cells, preferentially decreasing the first phase of exocytosis
M Ohara-Imaizumi, AK Cardozo, T Kikuta… - Journal of Biological …, 2004 - ASBMB
The prediabetic period in type I diabetes mellitus is characterized by the loss of first phase
insulin release. This might be due to islet infiltration mediated by mononuclear cells and
local release of cytokines, but the mechanisms involved are unknown. To determine the role
of cytokines in insulin exocytosis, we have presently utilized total internal reflection
fluorescence microscopy (TIRFM) to image and analyze the dynamic motion of single insulin
secretory granules near the plasma membrane in live β-cells exposed for 24 h to interleukin …
insulin release. This might be due to islet infiltration mediated by mononuclear cells and
local release of cytokines, but the mechanisms involved are unknown. To determine the role
of cytokines in insulin exocytosis, we have presently utilized total internal reflection
fluorescence microscopy (TIRFM) to image and analyze the dynamic motion of single insulin
secretory granules near the plasma membrane in live β-cells exposed for 24 h to interleukin …
