Human apoHpoprotetas AI, AII, CII and CIII. cDNA sequences and mRNA abundance

CR Sharpe, A Sidoli, CS Shelley… - Nucleic Acids …, 1984 - academic.oup.com
CR Sharpe, A Sidoli, CS Shelley, MA Lucero, CC Shoulders, FE Baralle
Nucleic Acids Research, 1984academic.oup.com
The structure and function of the genes encoding the polypeptide components of plasma
lipoproteins are of interest because of the central role they play in the regulation of lipid
metabolism. We have now completed our previous studies on the human apoAI gene and
furthermore isolated and sequenced cDNA clones for apoAII, CII and CIII. The nuoleotide
sequences show the signal peptides of apoAII, CII and CIII to be 18, 22 and 20 amino acids
in length, respectively, and in addition that prepro apoAII bears a classical propeptide …
Abstract
The structure and function of the genes encoding the polypeptide components of plasma lipoproteins are of interest because of the central role they play in the regulation of lipid metabolism. We have now completed our previous studies on the human apoAI gene and furthermore isolated and sequenced cDNA clones for apoAII, CII and CIII. The nuoleotide sequences show the signal peptides of apoAII, CII and CIII to be 18, 22 and 20 amino acids in length, respectively, and in addition that prepro apoAII bears a classical propeptide structure of 5 amino acids. The amino acid homology deteoted between apoCII and pro-apoAI is discussed, as is the gene arrangement of the 5′ non-coding region of apoAI mRNA. The relative liver mRNA levels of the 4 apolipoproteins analysed in this study have been estimated and compared with their corresponding plasma products. The data reported here provide an essential basis for further studies of structural and functional alleles of apo AI, AII, CII and CIII genes.
Oxford University Press