[HTML][HTML] Role of p97 AAA-ATPase in the retrotranslocation of the cholera toxin A1 chain, a non-ubiquitinated substrate
M Kothe, Y Ye, JS Wagner, HE De Luca, E Kern… - Journal of Biological …, 2005 - ASBMB
The enzymatic A1 chain of cholera toxin retrotranslocates across the endoplasmic reticulum
membrane into the cytosol, where it induces toxicity. Almost all other retrotranslocation
substrates are modified by the attachment of polyubiquitin chains and moved into the cytosol
by the ubiquitin-interacting p97 ATPase complex. The cholera toxin A1 chain, however, can
induce toxicity in the absence of ubiquitination, and the motive force that drives
retrotranslocation is not known. Here, we use adenovirus expressing dominant-negative …
membrane into the cytosol, where it induces toxicity. Almost all other retrotranslocation
substrates are modified by the attachment of polyubiquitin chains and moved into the cytosol
by the ubiquitin-interacting p97 ATPase complex. The cholera toxin A1 chain, however, can
induce toxicity in the absence of ubiquitination, and the motive force that drives
retrotranslocation is not known. Here, we use adenovirus expressing dominant-negative …