[HTML][HTML] The human–sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential
G Almeida-Porada, C Porada, N Gupta, A Torabi… - Experimental …, 2007 - Elsevier
OBJECTIVE: To investigate whether the sheep xenograft model of human hematopoiesis
can be used to mimic mobilization of human hematopoietic stem cells in vivo. MATERIAL
AND METHODS: Sheep transplanted with 3.6× 106 CD34+ from human adult bone marrow
were mobilized 1.5 years posttransplantation with human granulocyte colony-stimulating
factor for 5 days. At day 3 and 4 of mobilization, human cells were harvested from peripheral
blood (PB) and bone marrow (BM) and were injected into secondary sheep recipients (n= 6) …
can be used to mimic mobilization of human hematopoietic stem cells in vivo. MATERIAL
AND METHODS: Sheep transplanted with 3.6× 106 CD34+ from human adult bone marrow
were mobilized 1.5 years posttransplantation with human granulocyte colony-stimulating
factor for 5 days. At day 3 and 4 of mobilization, human cells were harvested from peripheral
blood (PB) and bone marrow (BM) and were injected into secondary sheep recipients (n= 6) …