Heterogeneity of airway inflammation in persistent asthma: evidence of neutrophilic inflammation and increased sputum interleukin-8
PG Gibson, JL Simpson, N Saltos - Chest, 2001 - Elsevier
Study objectives: To identify the characteristics of airway inflammation in persistent asthma
and to examine the role of neutrophilic inflammation in noneosinophilic persistent asthma.
Methods: Nonsmoking adults (n= 56) with persistent asthma and healthy control subjects (n=
8) underwent hypertonic saline solution challenge and sputum induction. Selected sputum
portions were dispersed with dithiothreitol and assayed for total cell count, cellular
differential, supernatant eosinophil cationic protein (ECP), myeloperoxidase, and interleukin …
and to examine the role of neutrophilic inflammation in noneosinophilic persistent asthma.
Methods: Nonsmoking adults (n= 56) with persistent asthma and healthy control subjects (n=
8) underwent hypertonic saline solution challenge and sputum induction. Selected sputum
portions were dispersed with dithiothreitol and assayed for total cell count, cellular
differential, supernatant eosinophil cationic protein (ECP), myeloperoxidase, and interleukin …