[HTML][HTML] Matrix GLA protein, a regulatory protein for bone morphogenetic protein-2
AF Zebboudj, M Imura, K Boström - Journal of Biological Chemistry, 2002 - ASBMB
Matrix GLA protein (MGP) has been identified as a calcification inhibitor in cartilage and
vasculature. Part of this effect may be attributed to its influence on osteoinductive activity of
bone morphogenetic protein-2 (BMP-2). To detect binding between MGP and BMP-2, we
performed immunoprecipitation using MGP and BMP-2 tagged with FLAG and c-Myc. The
results showed co-precipitation of BMP-2 with MGP. To quantify the effect of MGP on BMP-2
activity, we assayed for alkaline phosphatase activity and showed a dose-dependent effect …
vasculature. Part of this effect may be attributed to its influence on osteoinductive activity of
bone morphogenetic protein-2 (BMP-2). To detect binding between MGP and BMP-2, we
performed immunoprecipitation using MGP and BMP-2 tagged with FLAG and c-Myc. The
results showed co-precipitation of BMP-2 with MGP. To quantify the effect of MGP on BMP-2
activity, we assayed for alkaline phosphatase activity and showed a dose-dependent effect …