The glucokinase regulatory protein (GKRP) inhibits glucokinase competitively with respect to
glucose by forming a protein-protein complex with this enzyme. The physiological role of
GKRP in controlling hepatic glucokinase activity was addressed using gene targeting to
disrupt GKRP gene expression. Heterozygote and homozygote knockout mice have a
substantial decrease in hepatic glucokinase expression and enzymatic activity as measured
at saturating glucose concentrations when compared with wild-type mice, with no change in …