Alterations in the balance of cytoskeleton as well as energetic proteins are involved in the
cardiac remodeling occurring in dilated cardiomyopathy (DCM). We used two-dimensional
DIGE proteomics as a discovery approach to identify key molecular changes taking place in
a temporally controlled model of DCM triggered by cardiomyocyte-specific serum response
factor (SRF) knock-out in mice. We identified muscle creatine kinase (MCK) as the primary
down-regulated protein followed by α-actin and α-tropomyosin down-regulation leading to a …