Reaction rates of creatine kinase and ATP synthesis in the isolated rat heart. A 31P NMR magnetization transfer study.
JA Bittl, JS Ingwall - Journal of Biological Chemistry, 1985 - ASBMB
The NMR technique of magnetization transfer can be used to define intracellular reaction
kinetics. In order to determine the relationship between ATP synthesis and flux through the
creatine kinase reaction in the intact heart, we used this technique to measure flux through
the creatine kinase reaction in the isolated, isovolumic rat heart at five levels of cardiac
performance and oxygen consumption. The unidirectional reaction rate constants (s-1)
calculated from a two-site exchange model for both the forward and reverse creatine kinase …
kinetics. In order to determine the relationship between ATP synthesis and flux through the
creatine kinase reaction in the intact heart, we used this technique to measure flux through
the creatine kinase reaction in the isolated, isovolumic rat heart at five levels of cardiac
performance and oxygen consumption. The unidirectional reaction rate constants (s-1)
calculated from a two-site exchange model for both the forward and reverse creatine kinase …