[HTML][HTML] Kinetic, thermodynamic, and developmental consequences of deleting creatine kinase isoenzymes from the heart: reaction kinetics of the creatine kinase …
KW Saupe, M Spindler, JCA Hopkins, W Shen… - Journal of Biological …, 2000 - ASBMB
Creatine kinase (CK) exists as a family of isoenzymes in excitable tissue. We studied
isolated perfused hearts from mice lacking genes for either the main muscle isoform of CK
(M-CK) or both M-CK and the main mitochondrial isoform (Mt-CK) to determine 1) the
biological significance of CK isoenzyme shifts, 2) the necessity of maintaining a high CK
reaction rate, and 3) the role of CK isoenzymes in establishing the thermodynamics of ATP
hydrolysis. 31 P NMR was used to measure [ATP],[PCr],[P i],[ADP], pH, as well as the …
isolated perfused hearts from mice lacking genes for either the main muscle isoform of CK
(M-CK) or both M-CK and the main mitochondrial isoform (Mt-CK) to determine 1) the
biological significance of CK isoenzyme shifts, 2) the necessity of maintaining a high CK
reaction rate, and 3) the role of CK isoenzymes in establishing the thermodynamics of ATP
hydrolysis. 31 P NMR was used to measure [ATP],[PCr],[P i],[ADP], pH, as well as the …