[HTML][HTML] Demethylation of 3-methylthymine in DNA by bacterial and human DNA dioxygenases
P Koivisto, P Robins, T Lindahl, B Sedgwick - Journal of Biological …, 2004 - ASBMB
Rare DNA lesions that are chemically stable and refractory to repair may add
disproportionately to the accumulation of mutations in long lived cells. 3-Methylthymine is a
minor lesion that is induced by DNA-methylating agents and for which no repair process has
been described previously. Here we demonstrate that this lesion can be directly
demethylated in vitro by bacterial and human DNA dioxygenases. The Escherichia coli AlkB
and human ABH3 proteins repaired 3-methylthymine in both single-stranded and double …
disproportionately to the accumulation of mutations in long lived cells. 3-Methylthymine is a
minor lesion that is induced by DNA-methylating agents and for which no repair process has
been described previously. Here we demonstrate that this lesion can be directly
demethylated in vitro by bacterial and human DNA dioxygenases. The Escherichia coli AlkB
and human ABH3 proteins repaired 3-methylthymine in both single-stranded and double …