Interaction of human recombination proteins Rad51 and Rad54
EI Golub, OV Kovalenko, RC Gupta… - Nucleic acids …, 1997 - academic.oup.com
EI Golub, OV Kovalenko, RC Gupta, DC Ward, CM Radding
Nucleic acids research, 1997•academic.oup.comThe cDNA for human protein HsRad54, which is a structural homolog of Saccharomyces
cerevisiae recombination/repair protein Rad54, was cloned and expressed in Escherichia
coli. As demonstrated by analysis in vitro and in vivo, HsRad54 protein interacts with human
Rad51 recombinase. The interaction is mediated by the N-terminal domain of HsRad54
protein, which interacts with both free and DNA-bound HsRad51 protein.
cerevisiae recombination/repair protein Rad54, was cloned and expressed in Escherichia
coli. As demonstrated by analysis in vitro and in vivo, HsRad54 protein interacts with human
Rad51 recombinase. The interaction is mediated by the N-terminal domain of HsRad54
protein, which interacts with both free and DNA-bound HsRad51 protein.
Abstract
The cDNA for human protein HsRad54, which is a structural homolog of Saccharomyces cerevisiae recombination/repair protein Rad54, was cloned and expressed in Escherichia coli. As demonstrated by analysis in vitro and in vivo, HsRad54 protein interacts with human Rad51 recombinase. The interaction is mediated by the N-terminal domain of HsRad54 protein, which interacts with both free and DNA-bound HsRad51 protein.
Oxford University Press