Determination of the extracellular concentration of glycine in the rat spinal cord dorsal horn by quantitative microdialysis
KJ Whitehead, JP Manning, CGS Smith, NG Bowery - Brain research, 2001 - Elsevier
KJ Whitehead, JP Manning, CGS Smith, NG Bowery
Brain research, 2001•ElsevierTwo quantitative microdialysis methods were used to determine the extracellular
concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats.
Extracellular glycine determined by zero net flux was 2.6±0.3 μM and by the zero flow
method was 3.3±0.3 μM. For comparison the glycine content of cerebrospinal fluid was
determined to be 6.4±1.1 μM. There was no correlation between the extracellular and the
cerebrospinal fluid concentrations.
concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats.
Extracellular glycine determined by zero net flux was 2.6±0.3 μM and by the zero flow
method was 3.3±0.3 μM. For comparison the glycine content of cerebrospinal fluid was
determined to be 6.4±1.1 μM. There was no correlation between the extracellular and the
cerebrospinal fluid concentrations.
Two quantitative microdialysis methods were used to determine the extracellular concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats. Extracellular glycine determined by zero net flux was 2.6±0.3 μM and by the zero flow method was 3.3±0.3 μM. For comparison the glycine content of cerebrospinal fluid was determined to be 6.4±1.1 μM. There was no correlation between the extracellular and the cerebrospinal fluid concentrations.
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