[HTML][HTML] Ectopic expression of activated Stat6 induces the expression of Th2-specific cytokines and transcription factors in developing Th1 cells
H Kurata, HJ Lee, A O'Garra, N Arai - Immunity, 1999 - cell.com
H Kurata, HJ Lee, A O'Garra, N Arai
Immunity, 1999•cell.comStat6 is critical for IL-4-mediated Th2 cell development, but its molecular mechanism
remains unclear. Here we constructed Stat6: ER, a Stat6-estrogen receptor fusion protein
that can be activated by 4-hydroxytamoxifen, independently of IL-4 and endogenous Stat6.
Retrovirus-mediated introduction of Stat6: ER into developing Th1 cells induced Th2-specific
cytokines and suppressed IFNγ production in a 4-HT-dependent manner and in the absence
of IL-4. It also induced GATA-3 and c-maf expression and downregulated IL-12Rβ2 chain …
remains unclear. Here we constructed Stat6: ER, a Stat6-estrogen receptor fusion protein
that can be activated by 4-hydroxytamoxifen, independently of IL-4 and endogenous Stat6.
Retrovirus-mediated introduction of Stat6: ER into developing Th1 cells induced Th2-specific
cytokines and suppressed IFNγ production in a 4-HT-dependent manner and in the absence
of IL-4. It also induced GATA-3 and c-maf expression and downregulated IL-12Rβ2 chain …
Abstract
Stat6 is critical for IL-4-mediated Th2 cell development, but its molecular mechanism remains unclear. Here we constructed Stat6:ER, a Stat6-estrogen receptor fusion protein that can be activated by 4-hydroxytamoxifen, independently of IL-4 and endogenous Stat6. Retrovirus-mediated introduction of Stat6:ER into developing Th1 cells induced Th2-specific cytokines and suppressed IFNγ production in a 4-HT-dependent manner and in the absence of IL-4. It also induced GATA-3 and c-maf expression and downregulated IL-12Rβ2 chain expression. Its decreased ability to induce the Th2 phenotype with progressing Th1 cell commitment correlated with a decreased induction of GATA-3 and c-maf. This study indicates that Stat6 functions upstream of GATA-3 and c-Maf to induce Th2 development.
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