Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and …
AM Wobus, G Wallukat, J Hescheler - Differentiation, 1991 - Elsevier
A defined cultivation system was developed for the differentiation of pluripotent embryonic
stem cells of the mouse into spontaneously beating cardiomyocytes, allowing investigations
of chronotropic responses, as well as electrophysiological studies of different cardioactive
drugs in vitro. The β-adrenoceptor agonists (—) isoprenaline and clenbuterol, the mediators
of cAMP metabolism, forsko-lin and isobutylmethylxanthine (IBMX), the α 1-adreno-ceptor
agonist (—) phenylephrine, and the heart glyco-side digitoxine induced a positive, the …
stem cells of the mouse into spontaneously beating cardiomyocytes, allowing investigations
of chronotropic responses, as well as electrophysiological studies of different cardioactive
drugs in vitro. The β-adrenoceptor agonists (—) isoprenaline and clenbuterol, the mediators
of cAMP metabolism, forsko-lin and isobutylmethylxanthine (IBMX), the α 1-adreno-ceptor
agonist (—) phenylephrine, and the heart glyco-side digitoxine induced a positive, the …