MDM2 plays a major role in cancer development and progression via both p53-dependent
and-independent functions. One of its p53-independent functions is the induction of the
ubiquitin-independent proteasomal degradation of p21 Waf1. The present study was
designed to characterize the mechanism (s) by which MDM2 induces p21 Waf1 degradation.
We first determined the regions of MDM2 required for p21 Waf1 degradation using pulldown
assays and Western blotting and then examined the mechanisms using limited proteolysis …