[HTML][HTML] Palmitate increases sphingosine-1-phosphate in C2C12 myotubes via upregulation of sphingosine kinase message and activity [S]
W Hu, J Bielawski, F Samad, AH Merrill… - Journal of lipid …, 2009 - ASBMB
Studies in skeletal muscle demonstrate that elevation of plasma FFAs increases the
sphingolipid ceramide. We aimed to determine the impact of FFA oversupply on total
sphingolipid profiles in a skeletal muscle model. C2C12 myotubes were treated with
palmitate (PAL). Lipidomics analysis revealed pleiotropic effects of PAL on cell sphingolipids
not limited to ceramides. 13 C labeling demonstrated that PAL activated several branches of
sphingolipid synthesis by distinct mechanisms. Intriguingly, PAL increased sphingosine-1 …
sphingolipid ceramide. We aimed to determine the impact of FFA oversupply on total
sphingolipid profiles in a skeletal muscle model. C2C12 myotubes were treated with
palmitate (PAL). Lipidomics analysis revealed pleiotropic effects of PAL on cell sphingolipids
not limited to ceramides. 13 C labeling demonstrated that PAL activated several branches of
sphingolipid synthesis by distinct mechanisms. Intriguingly, PAL increased sphingosine-1 …